The 13 000 enhancer trap lines described here demonstrate that the gal4-gfp system functions equally well in rice, achieving a similar level of enhancer trapping efficiency as in arabidopsis, and represent a significant step towards establishing gal4 resources in the model monocot, rice. The gal4/uas system is a two-component gene expression system widely used for targeted gene misexpression the gal4-gfp enhancer trap lines used here . Gfp reports gal4-vp16 expression because gal4 controls transcription of gfp through the uas elements misexpression of gene x is achieved by super-transformation the uas:genex fusion to a line with an individual gal4 enhancer trap insertion. Gal4-gfp enhancer trap system it’s a system for gal4 targeted gene expression an enhancer trap vector bearing a modified gal4-vp16 gene was inserted randomly into .
A drosophila lexa enhancer-trap resource for developmental biology and neuroendocrine gal4 enhancer trap system in the stanex enhancer trap . Green fluorescent protein enhancer (genetics) the methodology employed for its specific expression—specifically the binary enhancer-trap p[gal4] system—as a . The system has two parts: the gal4 gene, encoding the yeast transcription activator protein gal4, and the uas (upstream activation sequence), an enhancer to which gal4 specifically binds to activate gene transcription.
Mapping and application of enhancer-trap flippase expression in the gal4/uas system has been widely and successfully used by drosophilists for various biological . We have carried out a gal4 enhancer trap screen in zebrafish, and have generated 184 stable transgenic lines with interesting expression patterns throughout the nervous system of these, three display clear expression in the tectum, each with a distinguishable and stereotyped distribution of gal4 . Over the past decade the adoption and refinement of the gal4 system by the drosophilafield has a gal4-mediated luc/gfp/gus enhancer trap system in .
Letter reﬁning gal4-driven transgene expression in drosophila with a gal80 enhancer-trap maximiliano l suster,1,2 laurent seugnet, 1michael bate, and marla b sokolowski2 1department of zoology, university of cambridge, cambridge,uk. Abstract to facilitate the monitoring of guard cells during development and isolation, a population of 704 gal4 gfp enhancer trap lines was screened and four. Development of a gal4-vp16/uas trans-activation system for tissue specific patterns in the gal4-vp16 system, al gal4-gfp enhancer trap lines for genetic . Transposition in drosophila melanogaster many other p-element lines to the gal4 system t he enhancer trap technique is a widely used for gfp visualization . Expression systems—such as the split-gal4 system 2 and qf-quas of enhancer trap and promoter fusion lines, but this limitation gal4 flp gfp c .
With the advent of the gal4 expression system, enhancer traps have a far greater potential for utility in biological studies gal4 enhancer trap driving uas-gfp . However, such a method had not been developed in zebrafish for a long time mainly because of the lack of an efficient transposon system such as the p transposon system in drosophila, by which a large number of insertions of a gal4 enhancer trap construct in different loci on the genome can be efficiently generated in this review, we will . The enhancer trap cassette consisted of a promoter-equipped gal4 gene adjacent to the right border and gfp fused to uas the cassette integrated into the rice genome was mediated via an agrobacterium this allowed the gal4 gene to be brought under transcriptional control of local genomic enhancers and promoters. Since expression of the responder is dependent on the gal4 pattern of the driver, brand and perrimon cleverly took advantage of the enormous diversity of genomic regulatory sequences and the vigor of the drosophila community by constructing an enhancer-trap gal4 construct (pgawb, fig 2).
The p[gal4] enhancer-trap technique: the p-element transposon of drosophila has provided us with an ideal mobilisable vector for the introduction of dna-constructs, encoding a wide variety of genes under various control elements into the germ-line (sentry and kaiser, 1992, finnegan, 1992). Gal4 enhancer trap technique developed by brand and perrimon (7) to target expression ofgfphereweshowthat gfp can be used as a vital marker of gene expression in a. Gal4-gfp enhancer trap lines for genetic manipulation of lateral root development in arabidopsis thaliana system is made of lateral roots that originate post-.